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https://www.eroids.com/reviews/geopeptides.com
The baculovirus expression vector system (BEVS) is a powerful system for the production of high quality proteins. Although time lines are increased compared to E. coli, proteins produced in BEVS tend to be soluble and functional and carry post-translational modifications like phosphorylation and glycosylation. The system gives very high recombinant protein yields and we often recommend it for proteins that are difficult to express in E. coli.
Phase I: Gene Synthesis, Subcloning, and Baculovirus Preparation
We can take a customer provided cDNA or optimize and synthesize any desired coding sequence and subclone it into a baculovirus transfer vector. Once cloned, all constructs are restriction digest and sequence verified. Transfer vectors are used to generate recombinant bacmids and P1, P2, and P3 virus stocks.
Phase II: Protein Expression and Purification
We infect insect cells with high titer P3 viral stock and determine optimal conditions for protein expression and purification.
Phase III: Larger Scale Protein Expression and Purification
Should additional quantities of protein be required we scale up expression and purification based on optimized conditions.
Purification Capabilities
Affinity Chromatography
Ni-NTA, Talon resin, Glutathione Sepharose, Heparin Sepharose, Streptavidin Sepharose, anti-Flag, Protein A, Protein G
Ion Exchange Chromatography
Mono-Q, Q Sepharose Fast Flow, Resource Q, Q Sepharose XL, ANX, DEAE Sepharose, SP Sepharose, CM Sepharose
Hydrophobic Interaction Chromatography
Phenyl and (CH2)n Sepharose
Size Exclusion Chromatography
Additional Services
Tag removal
Endotoxin Removal
Protein solublization and refolding
Metabolic labeling: Stable isotope labeling with 13C and 15N.
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