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Protein Services - Baculovirus System

CHECK US OUT AND VOTE FOR US!!

https://www.eroids.com/reviews/geopeptides.com

 

The baculovirus expression vector system (BEVS) is a powerful system for the production of high quality proteins. Although time lines are increased compared to E. coli, proteins produced in BEVS tend to be soluble and functional and carry post-translational modifications like phosphorylation and glycosylation. The system gives very high recombinant protein yields and we often recommend it for proteins that are difficult to express in E. coli. 

Phase I: Gene Synthesis, Subcloning, and Baculovirus Preparation
We can take a customer provided cDNA or optimize and synthesize any desired coding sequence and subclone it into a baculovirus transfer vector. Once cloned, all constructs are restriction digest and sequence verified. Transfer vectors are used to generate recombinant bacmids and P1, P2, and P3 virus stocks.

Phase II: Protein Expression and Purification
We infect insect cells with high titer P3 viral stock and determine optimal conditions for protein expression and purification.

Phase III: Larger Scale Protein Expression and Purification 
Should additional quantities of protein be required we scale up expression and purification based on optimized conditions.

 

Purification Capabilities

Affinity Chromatography
Ni-NTA, Talon resin, Glutathione Sepharose, Heparin Sepharose, Streptavidin Sepharose, anti-Flag, Protein A, Protein G
Ion Exchange Chromatography
Mono-Q, Q Sepharose Fast Flow, Resource Q, Q Sepharose XL, ANX, DEAE Sepharose, SP Sepharose, CM Sepharose
Hydrophobic Interaction Chromatography
Phenyl and (CH2)n Sepharose
Size Exclusion Chromatography

Additional Services
Tag removal
Endotoxin Removal
Protein solublization and refolding
Metabolic labeling: Stable isotope labeling with 13C and 15N.

Quotation and Ordering

  • For quotation requests and questions, you may contact us by email with completed form below.
  • Orders can be placed by email or online with a formal Purchase Order or credit card.
  • Once quote is recieved via email we will provide you were to send you samples to.

Our customer service is available 24hours per day/7 days a week/365 days a year.

Protein Service Quotation Request Form
    For entering sequences, please use amino acid codes starting with the N-terminal and ending with the C-terminal
Alanine
Arginine
Asparagine 
Aspartic Acid 
Cysteine
Glutamic Acid 
Glutamine
Ala (A)
Arg (R)
Asn (N)
Asp (D)
Cys (C)
Glu (E)
Gln (Q)
Glycine
Histidine
Isoleucine
Leucine
Lysine
Methionine
Phenylalanine
Gly (G)
His (H)
Ile (I)
Leu (L)
Lys (K)
Met (M)
Phe (F)
Proline
Serine
Threonine
Tryptophan
Tyrosine
Valine
Pro (P)
Ser (S)
Thr (T)
Trp (W)
Tyr (Y)
Val (V)
 
Protein Name Express System:Bacteria Yeast Baculovirus / Insect Cell Mammalian Cell
Sequence:
(Protein or DNA)
Start From: Gene SynthesisSubcloning with your cDNA  Your supplied expression ready vector
Comments:
PLEASE PRINT OUT THE ABOVE FORM, SCAN AND EMAIL TO [email protected]